Friska Research Lab

We are proud to have a state-of-the-art Research Lab having International Accreditations with capability of Chromosome Profiling, FISH, Micro arrays, Genomics, Cell Harvesting etc. We have implemented top quality management parameters as per best international guidelines. The lab is ISO/IEC 17025, ISO 15189, ISO 13485 certified and NABL compliant. It is under the supervision of the following competent scientist. 

The lab is equipped in DNA and RNA extraction, isolation, purification and analysis. It has state of the art equipment for Plasmid DNA Isolation, Genomic DNA Extraction, DNA Electrophoresis, DNA Ladders, DNA quantitation and Nucleic Acid Labelling. We are using the latest generation scientific equipment from ThermoFisher for our oncological research and analysis. Few of the equipments used by us are as under:

Ion PGM Sequencer

AmpliSeq Panels

QuantStudio 12k Flex Real-Time PCR System

TaqMan® Assays

3500 Genetic Analyzer

Sanger sequencing

Somatic Mutation Detection

TaqMan Mutation Detection Technology—Offering Superior Sensitivity for Your Cancer Biomarker Research
TaqMan Mutation Detection Assays detect somatic mutations in genes that are associated with cancer. These research use only assays are powered by competitive allele-specific TaqMan PCR technology, also known as castPCR technology. The castPCR technology is highly specific and sensitive, and it can detect rare amounts of mutated DNA in a sample that contains large amounts of normal, wild type DNA. These assays are compatible with different sample types, such as cell lines, FFPE tissue samples, and fresh frozen tissue samples.

  • High specificity—mutant allele detection is based on an allele-specific primer, while the wild-type background is suppressed by an MGB blocker oligonucleotide
  • High sensitivity—assays can detect as low as 0.1% mutant molecules in a background of wild type gDNA as demonstrated in spiking experiments
  • Wide dynamic range and excellent PCR efficiency—assays demonstrate at least 4 logs of dynamic range and an average efficiency of 100 ± 10%
  • Fast, simple workflow—like other TaqMan Assays, typically requires 3 hours from sample to results, with minimum hands-on time

Ion GeneStudio™ S5 Prime System

The Ion GeneStudio S5 Prime System is a semiconductor-based next-generation sequencing (NGS) system that enables simple targeted sequencing workflows. For core and service labs requiring maximum throughput and rapid turnaround time from a benchtop NGS system, the Ion GeneStudio S5 Prime System enables unparalleled breadth and depth, from large panels or exomes to clinical oncology research focused on low allele frequency applications. With cartridge-based reagents, the system is simple to use and offers scalability and flexibility. It uses Ion 5 Series chips to support a broad range of high-throughput sequencing for clinical research and research applications from microbial genomes and gene panels to exomes and transcriptomes.

1. Chief Scientist and Research Lab In-charge

Dr. L.K. Sahu

Research Scientist 

Clinical cytogenetics

Ph.D, National Institute of Immunology, New Delhi, India.

Advanced Course in Bioinformatics and Integrated Genomics from Harvard University , USA

Advanced Course in Cancer Genomics and Precision Oncology from Harvard University , USA

Expertise in Pharmacogenomics, Cytogenetics, FISH, Chromosome Analysis, Chromosome Profiling  & Microarray Test as per ISO 15189:2012, CAP and other International Guidelines. Renowned scholar and acclaimed scientist in DNA Technology, Gene Expressions and Functional Genomics, with more than 22 years of Global Experience.

2. Associate Scientist

Dr. Phil Harvey

Research Scientist

Clinical Cytogenetics

Ph.D, Human Genetics, McGill University, Montreal, Canada.

Renowned scholar and acclaimed scientist in DNA Technology, Gene Expressions and Functional Genomics, with more than 12 years of Global Experience.

3. Associate Lab In-charge, Friska Lab (India)

Dr. D. M. Malhotra

Research Scientist

Ph.D, National Institute of Immunology, New Delhi, India.

Executive Assistant with an experience of 11 years in specimen preparation, standard operating procedures and reporting as per NABH, NABL, CAP and other International Guidelines